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However, in these studies the binding was considered as unspecific and non-covalent, and not linked to a physiological function.
The presence of GO terms "sequence-specific DNA binding transcription factor activity" "transcription regulator activity", "bHLH transcription factor binding", "transcription activator activity", "DNA binding" was considered as an evidence that the protein is a transcription factor or is involved in some way in transcription regulation.
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CTCF binding is considered as a mark for potential insulator elements (Kim et al. 2007).
Differentially expressed genes were considered as nodes, and processes and binding were considered as edges that connect the nodes.
Here, we measure prediction accuracy conservatively: all disordered residues not annotated as protein binding were considered as not being able to do so, even under different physiological conditions e.g. in the presence of other proteins.
In Raji cells, the immunoreactivity of 111In-DOTA-belatacept was 28.3 % and binding was considered specific as incubation with 0.1 mM unlabeled belatacept resulted in a reduced radiotracer binding (Fig. 1c).
But some legal experts said that the governor would have a better chance of winning a court fight over layoffs, because the deal with unions was not part of a collective bargaining agreement and might not be considered as binding as if it had been included in a labor contract.
In the present study, search was performed on the global surface of the protein by similarity and substructure searching [ 52], and the automatic identification of binding sphere was considered as highly significant.
The binding to native LDL was considered as nonspecific binding.
In case of the equilibrium binding studies with the GpL fusion proteins of the various TRAIL variants and Hek293 cells transiently transfected with the various TRAIL receptors, binding to cells transfected with empty vector was considered as nonspecific binding due to the low expression of the endogenous receptors.
Thus, Bodin's sovereign was restricted by the constitutional law of the state and by the higher law that was considered as binding upon every human being.
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