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Plaque binding was confirmed by thioflavin-S staining.
Cell binding was confirmed by visualizing adherent cells by fluorescence microscopy.
The EstC-NH2 grafting on the thiol MUA spacer through the formation of an amide binding was confirmed by the PM-RAIRS and XPS complementary experiments.
The specificity of the binding was confirmed by inhibition studies, which showed reduced crosslinking signal after pre-incubation of C3 with compstatin but not with various inactive analogs.
The essential role of Phe313 and Trp305 in N-6 binding was confirmed by our SPR assays, showing that N-6 had much lower binding affinity to two RXRα-LBD point mutants with Ala substitution of Phe313 or Trp305 (Fig. 4D and 4E).
The in vitro binding was confirmed by overlay of the blotted membranes with radio-labeled HAMLET (Figure 2A, right panel).
Similar(26)
For the dissociation rate constant analysis, complete initial binding is confirmed by a gel shift, and then a 100-fold excess of unlabeled DNA 24-mer is added.
The importance of electrostatics in MBD binding is confirmed by the ability of the poly dA-dT) compoly dA-dT ameliorate the influencompetitor.
The amino acid residues of the T β4 protein that are critical for action binding were confirmed by chemical cross-linking and shown to correspond to a hydrophobic cluster consisting of residues M6-I9-F12 M6-I9-F12 M6-I9-F12elin, as well as K14 and K18 [ 7].
The quality of phage deposition was monitored by ELISA, and phage bacterial-binding was confirmed by high-phage bacterial-bindingtrast microscopy.
The specificity of the binding was confirmed and validated by both fluorescence-activated cell sorting (FACS) and surface plasmon resonance (SPR) assays in different experimental setups.
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