Sentence examples for binding was conducted at from inspiring English sources
The calcium binding experiments were conducted at 37 °C, and lipid binding was conducted at 30 °C.
Antibody binding was conducted at 4 °C overnight and proteins were visualized using an enhanced chemiluminescence reagent and the ImageQuant LAS4000 system (GE Healthcare, Piscataway, NJ, USA).
Cells were plated and treated as before mentioned, the Annexin V binding assay was conducted at 24, 48 and 72 h post-irradiation.
The binding reaction was conducted at room temperature for 20 min with 5 μg of nuclear extract, 40,000 dpm (0.08 0.4 ng) of radiolabeled oligonucleotide probe, in 1x Ficoll buffer (10 mM Tris (pH 7.5), 1 mM DTT, 1 mM EDTA, and 4% Ficoll), 250 ng of poly(deoxyinosinic-deoxycytidylic acid) in 75 mM KCl, and double-distilled H2O to make the volume to 15 μl.
The saturation binding assay for GST-ERR-γ –LBD was conducted at 4°C using [H]BPA (5 Ci/mmol; Moravek Biochemicals, Brea, CA, USA) with or without BPA (10 μM in the final solution).
Finally docking of 1809 molecules was conducted at the IL-1β receptor binding site using MOE and FRED docking program.
