Sentence examples for binding was competed with from inspiring English sources

After washing to remove unbound protein, binding was competed with 50mM imidazole to remove nonspecific proteins and then CaRF was eluted with 250mM imidazole.

After the binding was competed with increasing amounts of an unlabelled oligonucleotide corresponding to allele C, the inverse of the intensity of the major band was plotted against the excess of unlabelled oligonucleotide.

In some reactions binding was competed with excess ATP using a regeneration system consisting of 2 mM ATP, 10 mM creatine phosphate disodium salt, 3.5 U/mL creatine kinase and 0.6 U/mL inorganic pyrophosphatase.

Incubation of PAE/VEGFR-2 and PAE/VEGFR-1 cells with I-VEGF121/rGel demonstrated binding specificity that was competed with unlabeled VEGF121/rGel but not with unlabeled gelonin.

The Sryα binding is competed off with unlabeled Sry DNA (lane 7).

The binding to the −151T probe was competed with 200-fold molar excess of unlabeled probe.

The binding of steroid-binding proteins to the testosterone-BSA surface was competed with free steroid hormone molecules (testosterone, DHEAS, androstenedione, estradiol, and DHT (Steraloids Inc., USA)) and free biotin (Biochemica, Fluka, 14400) to evaluate the specificity of binding.

The binding of scFvs to biotinylated-PLAP was competed with the increasing molar concentrations of unbiotinylated PLAP, IAP or BAP.

Moreover, EMSAs showed that CHES-1-like bound specifically to all three of the wild-type Fkh-binding sites in vitro, and that this binding could be competed with the wild type but not with the mutated binding sites (Fig. 1F), results that further strengthen this hypothesis.

Furthermore, EMSAs showed that Bin bound specifically to all three of the wild-type Fkh motifs in vitro, and that this binding could be competed with the wild type but not with the mutated binding sites (Fig. 1E), results that correlated with the effect of mutated Fkh sites on enhancer activity.

Binding, as measured by surface plasmon resonance (SPR) on a BIAcore1000 (BIAcore, Piscataway, NJ, USA), requires the presence of both the peptide and SA; binding cannot be competed with either of the components alone.