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Color compensation of phycoerythrin and fluorescein isothiocyanate was done with anti-mouse immunoglobulin, κ/negative control compensation particle set (BD Biosciences. Nonspecific binding was checked with IgG1 isotype controls (Beckman Coulter).
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Specificity of Annexin-V binding was checked by incubating MPs in calcium-free PBS supplemented with 0.32% citrate.
The binding specificity of these anti-V3 scFvs was checked with phage from the 24 well plate and PEG precipitated purified phage.
Nattokinase production was checked with fibrinolytic activity.
Immiscibility was checked with thermal data.
Transfer efficiency was checked with Ponceau staining.
Read qualities was checked with FASTQC [ 50].
Integrity was checked with an agarose gel.
Reproducibility was checked with positive controls.
Integrity was checked with agarose gel electrophoresis.
These models are checked with analytical formulations.
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