Exact(3)
Specificity of Annexin-V binding was checked by incubating MPs in calcium-free PBS supplemented with 0.32% citrate.
Specificity of binding was checked by incubating in the presence of an excess of 'cold' NF- κB oligo sequence.
Specificity of antibody binding was checked by treating cryostat sections in the described fashion except that primary antibodies were omitted from the procedure.
Similar(57)
Mutation of the binding consensus site was checked by sequencing, and the fragment was purified by the silica-based Gene Clean II kit (MP Biomedicals, Cleveland, OH) and ammonium acetate DNA precipitation method.
The specificity of the C-542 antibody was checked by comparing its binding with the nonspecific binding of mouse IgG1k (mIgG1k, of the same immunoglobulin isotype) and by the ability of the peptide representing the C-542 epitope (Genosys, Woodlands, TX, USA) to decrease C-542 binding.
RelA/p65 specific siRNA knockdown, whose efficacy was checked by immunoblotting, reduced RelA/p65 binding activity by ~50%.
The cooperativity of mutations was checked by comparing the changes of experimental binding affinities (ΔΔ G) for the seven mutants in the series.
The successful binding was visualized by EM and AFM measurements, and the functioning of the RC was checked by flash photolysis experiments.
Specificity was checked by measuring the ability of soluble wild-type or mutated oligonucleotides to inhibit binding.
Purity was checked by HPLC.
Coding was checked by SD.
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