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Exact(9)
Percent of binding was calculated from the resulting number of pixels for each of the investigated factors.
Specific binding was calculated from the difference between the integrated radioactivity signals per area (DLU/mm2) under baseline and blocking conditions, divided by the value under baseline conditions.
Percent of gp120-CD4 binding was calculated from gp120-CD4 complex formation in the absence of any inhibitor.
Absorbance was measured at 415 nm and relative binding was calculated from background-subtracted values.
Specific binding was calculated from the difference between radioactivity levels measured in the absence and presence of unlabelled oestradiol.
As a consequence, the apparent second-order rate (kon) constant for cyanide binding was calculated from the slope of the linear plot of kobs(1) versus cyanide concentration.
Similar(51)
Changes in free energy (ΔG) and entropy (ΔS) upon binding were calculated from the KD and ΔH as described in Methods.
The apparent dissociation constants for hematein binding were calculated from secondary plots of the slopes or intercepts obtained from the Linewear-Burk plots.
The percentage binding was calculated for each dilution and averaged.
The degree of binding was calculated by taking the theoretical concentrations calculated from the proteins added to the silica particles originally as 100%.
Specific binding was calculated by subtracting nonspecific from total binding at each concentration of radioligand.
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