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Specific binding was calculated by subtracting nonspecific from total binding at each concentration of radioligand.
Specific binding was calculated by subtracting the non-specific binding (111In-DOTA-belatacept and excess unlabeled belatacept) from the total binding (111In-DOTA-belatacept only).
The degree of binding was calculated by taking the theoretical concentrations calculated from the proteins added to the silica particles originally as 100%.
The specific binding was calculated by subtraction of the non-specific binding from the total binding.
Specific binding was calculated by subtracting the non-specific binding from the total binding.
The specific binding was calculated by the total binding (cpm) - nonspecific binding (cpm).
Similar(36)
The EC50 titers, representing the concentration for 50% of maximal binding, were calculated by GraphPad Prism.
For endogenous Myc binding, triplicate sets of ChIP and input DNA were applied on this custom array and genomic sites enriched for c-Myc binding were calculated by ChIP versus input (log2 ratio provided by NimbleGen data output).
Tm, enthalpy of unfolding (ΔHu), and change in heat capacity on binding were calculated by fitting the experimental data to equations 9, 24, and 25 from Layton and Hellinga (2010).
The OD intensity reduction caused by serum antibodies blocking mAb binding was calculated for each sample dilution by using the formula: % inhibition = [ negative reference serum OD−test serum OD)/(negative reference serum OD−positive reference serum OD)]×100%.
For qPCR of ChIP analysis, one primer set was used for each tested binding region (Supplementary Table S18) and ChIP efficiency of each binding site was calculated by comparison of the percentage of ChIPped DNA against input chromatin to this percentage at a negative control region.
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