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Specific binding was calculated as total minus nonspecific binding performed in the presence of cold SCH23390 (20 µM).
Specific binding was calculated as the difference between total and nonspecific binding measured in parallel assays.
Lysine-dependent plg binding was calculated as total plg binding minus binding in the presence of 1 mM tranexamic acid.
The value of cross-reacting antibody binding was calculated as the total HI binding that was inhibited by IAsp.
The extent of RCA-I binding was calculated as the ratio of positive cells to total cancer cells, and a value of 10% or higher was defined as a positive signal [ 2].
Mitochondrial binding was calculated as the proportion of the labeled peptide (input TnT, first lane of each panel) able to be pulled down or imported into either control or uncoupled mitochondria (second and fifth lane of each panel, respectively).
Similar(48)
Asymmetry indices (AIs) of FP-CIT binding were calculated as reported by Sherfler and colleagues [37].
Dissociation off-rate constants assuming first-order 1 1 Langmuir binding are calculated as kd with IAA = 1.09 × 10 3 s–1, and kd without IAA = 5.58 × 10 3 s–1 (X = 1.07).
The cumulative binding was calculated using equation (2) as described previously.
The bimolecular rate of dNTP binding was calculated by pseudo-first-order approximation as the slope of this linear concentration dependence.
Specific binding was calculated by subtracting non-specific binding – defined as that seen in the presence of 1 μM unlabelled GABA or 1 μM diazepam or chlordiazepoxide – from the total binding obtained with radioligand alone.
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