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Non-specific binding was blocked with PBS containing 5% goat serum for 30 min at RT.
Non-specific binding was blocked with 5% Milk-TBS for 1 hour at room temperature.
Non-specific binding was blocked with 5 10% goat serum for 60 minutes.
Nonspecific binding was blocked with 10% horse serum in 3% BSA.
Non-specific antibody binding was blocked with 5% normal goat serum (NGS) in PBS for 1 h at room temperature.
Slides were fixed with cold acetone and non-specific binding was blocked with image-iT FX signal enhancer (Invitrogen).
Non-specific binding was blocked with 20% BSA in WB for 30 min at 37°C while shaking.
Nonspecific binding was blocked with 10% nonimmune serum of a species from which the secondary antibody had been obtained.
Non-specific binding was blocked with phosphate buffered saline (PBS) containing 2% gelatin and 3% bovine serum albumin.
Sections were permeabilized with PBS/0.2% Tween, and unspecific antibody binding was blocked with 5% horse serum.
Nonspecific antibody binding was blocked with 5% BSA in Tris-buffered saline (TBS) for 1 h at room temperature.
More suggestions(15)
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