Sentence examples for binding was blocked using from inspiring English sources

Nonspecific protein binding was blocked using a 5% milk solution at 4°C overnight.

Non-specific binding was blocked using 5% (v/v) normal sheep serum for 30 min at 37°C.

Non-specific protein binding was blocked using normal goat serum and endogenous peroxidase activity was quenched using 0.3% H2O2 in methanol prior to application of the primary antibody.

Non-specific binding was blocked using 3% bovine serum albumin (BSA) in phosphate-buffered saline (PBS) for 1 hour at room temperature (RT) before incubation with ligands.

Non-specific binding was blocked using 2% low fat dried milk (marvel) in PBST (blocking buffer) at 37°C for 2 hours.

Briefly, slides were dried, fixed in 4% paraformaldehyde for 15 minutes and non-specific antibody binding was blocked using 10% normal goat serum.

Retrieval were performed by proteinase K 2μg/ml (Promega Corporation, Madison, USA) for 5 min at room temperature and nonspecific binding were blocked using 10%% new calf serum and 10%% blocking reagent (Sigma).

Nonspecific binding of IgG was blocked using Ultra V block for 10 min at room temperature.

Aspecific binding of antibodies was blocked using 10%% normal goat serum (DAKO, Heverlee, Belgium) in PBS for 30 min at room temperature.

Nonspecific protein binding on the membranes was blocked using Tris-buffered saline with 0.05% Tween 20 (TBST) and 10% non-fat dry milk (blocking buffer).

Non-specific binding and endogenous peroxidase activity was blocked using 10% casein and 0.3%H2O22, respectively.