Sentence examples for binding was blocked in from inspiring English sources

Non-specific binding was blocked in 1% BSA in PBS, followed by incubation with anti-MMP-2 and anti-p65 antibodies for 2 h at room temperature.

Equal protein lysate was run on SDS-polyacrylamide (4 12%) gels, transferred onto polyvinylidene fluoride membranes (PVDF) and non-specific binding was blocked in TBS containing 5% non-fat powdered milk and 0.1% Tween-20at room temperature.

Brain sections were mounted onto Colorfrost®/Plus slides (Fisher Scientific) Non-specific binding was blocked in NGS (10% normal goat serum, 0.2% Triton X-100, and 0.02% NaN3 in Tris-buffered saline (TBS)) or in NGS without detergents for non-permeabilizing experiments, for 120 min at room temperature.

Equal protein lysates were run on SDS-polyacrylamide (10%) reducing gels, transferred onto polyvinylidene fluoride membranes (PVDF) and non-specific binding was blocked in tris buffered saline (TBS) containing 5% non-fat powdered milk and 0.05% Tween-20 at 4°C.

Unspecific binding was blocked in blocking buffer (LICOR).

Nonspecific binding was blocked in tris-buffered saline containing 5% nonfat dry milk before incubation with the primary antibodies.

The endogenous peroxidase activity was quenched with 3%H2O22 in deionized water for 10 min. Non-specific bindings were blocked in TBS containing 5% fraction V bovine albumin (Sigma-Aldrich, St . Louis MO, USA), 5% normal mouse serum (Dako Corp., Carpinteria, CA) and 0.1% porcine gelatin type B (Sigma-Aldrich).

The endogenous peroxidase activity was quenched with 3%H2O22 in deionized water for 10 min. Non-specific bindings were blocked in TBS containing 5% acetylated albumin (Aurion, Wegeningen, The Netherlands), 5% normal mouse serum (Dako Corp., Carpinteria, CA) and 0.1% porcine gelatin type B (Sigma-Aldrich).

For AR and Nkx3.1 staining, nonspecific binding was blocked by incubating in 1% bovine serum albumin in Tris-HCl pH 7.5 for 20 min at room temperature.

Non-specific binding was blocked by incubation in 2% normal swine serum (Dako) in phosphate-buffered saline.

Non-specific binding was blocked by incubation in 5% non-fat dry milk in PBS pH 7.5 and incubation in normal goat serum.