Sentence examples for binding was blocked for from inspiring English sources

Non-specific antibody binding was blocked for 20 minutes by incubation with 10% normal rabbit serum.

Briefly, nonspecific binding was blocked for 30 minutes with 4% horse serum or 4% goat serum in phosphate-buffered saline.

The non-specific binding was blocked for 30 minutes with either 5% goat serum (DakoCytomation) for the primary rabbit polyclonal antibodies or 5% rabbit serum (DakoCytomation) for the primary mouse monoclonal antibodies and primary antibody incubation was performed at 4°C overnight.

For IHC, hybridized slides were equilibrated in maleic acid buffer, pH 7.5 (100 mM maleic acid, 150 mM NaCl, and 0.1% Tween 20) and non-specific antibody binding was blocked for 2 hrs in maleic acid buffer containing 1% non-fat dry milk.

The cells (2×106) were washed once with FACS buffer (1% FBS and 0.1% Na-azide in PBS) and nonspecific binding was blocked for 10 minutes on ice using rat anti-mouse CD16/CD32 antibody in FACS buffer (clone 2.4 G2, BD PharMingen).

Non-specific binding was blocked for 10 min using protein-blocking buffer.

Unspecific bindings were blocked for 2 h in the corresponding serum and then the slices were incubated with the primary antibodies and stored at 4°C overnight.

Non-specific binding was blocked by incubation for 5 h in PBS containing 3% BSA.

In the preadsorption experiments, antibody binding was blocked by preincubation for 5 h at 4°C with a 20-fold molar excess of the peptide.

Nonspecific binding was blocked through incubation for 30 min with 3% BSA.

Non-specific binding was blocked by incubation for 30 min in 4% normal goat serum and 1% BSA in PBS.