The non-specific binding was blocked by goat serum (Zhongshan Golden Bridge Biotechnology, #ZLI-9021) at room temperature for 2 h.
The fixed cells were permeabilized with 0.3% Triton X-100, and nonspecific binding was blocked by using 1% normal goat serum.
CD44v6 levels returned to control levels when OPN-alphavbeta3-integrin binding was blocked by an RGD peptide or tyrosine kinase activity was inhibited.
RV144 vaccinee serum IgGs bound more avidly to A244 gp120 Δ11 than to the unmodified gp120, and their binding was blocked by C1, V2, and V1/V2 antibodies.
Non-specific Ab binding was blocked by incubation in 20% normal goat serum (DAKO, France).
This binding was blocked by an anti-mouse CD40 monoclonal antibody (Fig. 4C).
Nonspecific protein binding was blocked by 5% FCS (Gibco, Karlsruhe, Germany).
Non-specific Ab binding was blocked by incubation in 20% normal donkey serum (DAKO, France).
Non-specific Ab binding was blocked by incubation in 20% normal mouse or goat serum (DAKO).
Non-specific binding was blocked by incubation for 5 h in PBS containing 3% BSA.
Unspecific binding was blocked by 5% milk in PBS-0.1% Tween for 2 hours.
