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Non-specific binding was avoided by saturating samples with PBS/4% BSA.
Non-specific binding was avoided by blocking the PVDF membrane with 5% skimmed milk in TBS-T for 1 h.
Paraffin embedding was removed from the microarray slides and after rehydration they were subjected to antigen retrieval in a microwave oven (600 W) employing Tris-EDTA buffer followed by 3 wash cycles with TBS buffer for 5 min. Unspecific binding was avoided by adding 5% TBS/BSA for 1 h at room temperature.
Similar(57)
Non-specific binding of the antibodies was avoided by blocking with 5 % BSA in PBS for 30 min, followed by incubation with primary antibodies of CK (1 50) and VIM (1 50) overnight at 4 °C, and then with secondary antibodies for 1 h at 37 °C.
On the other hand, it is conceivable that in some cases, immobilisation of the ligand directly to the microwell may cause a sterical hindrance that interferes with binding of recombinant phage, which can be avoided by biopanning.
Hammering is avoided by force-controlled stepping.
Being avoided by Taylor Swift, Mayerr said.
These are avoided by MTS. 2).
You may be avoided by peers.
While highly conserved regions as primer binding sites were avoided to prevent amplification of human DNA, less conserved regions as primer-binding sites may explain the lack of detection of certain mucorales by the ITS-2 assay.
Can the tired, old, and exploitive devices e.g. shots of the person putting on makeup, shopping for shoes, getting dressed, binding, etc., be avoided?
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