Sentence examples for binding was assessed with from inspiring English sources

Non-specific binding was assessed with co-incubation with 10 μM PK11195 (Sigma-Aldrich).

[35S]GTPγS-stimulated binding was assessed with NXT TOPCOUNTER and expressed as a percentage increase in basal [35S]GTPγS-binding.

Protein binding was assessed with hyperimmune anti-FN serum at the dilution of 1 : 100 followed by incubation with HRP-conjugated anti-mouse IgG (Sigma) (1 : 500).

Flow-cytometric assessment of C3 binding was detected with fluorescein isothiocyanate-conjugated anti-C3 (1:40; DAKO), and C9 binding was assessed with mouse anti-human C5b-C9 (Technoclone, Vienna, Austria).

Binding was assessed with both yeast actin and rabbit muscle actin.

Antibody binding was assessed by incubation with horseradish peroxidase-conjugated antimouse secondary antibodies (Santa Cruz Biotechnology), BMI1 (Cell Signaling Technologies, Beverly, MA, USA) and E2F1 (Upstate, MA, USA).

AML1 and AML1-MTG16 binding was assessed by ChIP with antibodies against either the AML1 C-terminus (Santa Cruz Biotechnology, Santa Cruz, CA, USA), or the MTG16 C-terminus [ 28], respectively.

Ubiquitin binding was assessed by pull-down with Dynabeads® and blotting with antibodies against the HIS6 and FLAG tags in both the absence and presence of β-mercaptoethanol (which reduces thiolester bonds).

Since the selectivity of a PET tracer for its target is of crucial importance, THK5117 binding was assessed in competition studies with other compounds.

Nonspecific binding was assessed by coincubating the slides with 20  μM of either PK11195 or CLINME.

Affinity of agonist binding was assessed indirectly in competition experiments with 1 nM of the muscarinic radioligand [H]NMS.