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Plasma protein binding was assessed using ultrafiltration.
LDL-proteoglycan binding was assessed using surface plasmon resonance.
MHC class I expression and BNP Ab binding was assessed using flow cytometry (a,b) and confocal imaging (c).
In addition, specific binding was assessed using autoradiography on ALK5 expressing MDA-MB-231 tumor sections.
AV-45 binding was assessed using an innovative extraction method allowing quantifying uptake in the cortex only.
The total amount of protein binding was assessed using a scintillation counter and results were recorded as counts per minute and compared as a percentage to binding of wildtype Munc18-1 protein.
Similar(39)
Interobserver variability in the hypothalamic specific-to-nonspecific binding ratios was assessed using intraclass correlation coefficient (ICC).
TSPO binding density was assessed using the selective TSPO ligands, 125I]-CLINDE and 18 F]-PBR111 as described previously [9, 23].
Nrf2 DNA binding activity was assessed using a TransAM Transcription Factor ELISA kit (Active Motif).
The quality of the p38α binding assay was assessed using the method of Zhang et al. [ 25].
Finally, statistical significance of the spatial clustering of positively selected amino acids and correspondence with functional binding sites was assessed using programs from Clark and Swanson [ 78].
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