Sentence examples for binding was assessed in from inspiring English sources
Non-specific binding was assessed in parallel pull down assays employing mock-transfected Bm5 cell lysates.
BCO mimic binding was assessed in the presence of 25 nM each DNO (Figure 4E & F).
In order to reduce the number of confounding physiological variables associated with live cell cultures, peptide binding was assessed in purified membrane preparations, but surprisingly, no comparable displacement of [125I]α-BTX binding was detected.
Additionally, patients' serum IgE binding was assessed in ELISA experiments including Bet v 1 isoform 0101, which was demonstrated to be suitable as reference material for analysis of IgE binding to Bet v 1 [27], as positive control.
Total binding was assessed in the absence of unlabelled peptide.
Nonspecific binding was assessed in coverslips that were labeled with secondary antibody only.
Affinity of agonist binding was assessed indirectly in competition experiments with 1 nM of the muscarinic radioligand [H]NMS.
The in vitro cell binding was assessed by growing B16F1 cells in culture flasks to 80 90% confluence, trypsinizing, washing in PBS (pH 7.4), aliquoting into 12-well plates and allowing adherence to the wells overnight.
TSPO binding was assessed as described above in the cortex, corpus callosum, hippocampus and hippocampal subregions (CA1, CA2 and CA3/hilus), thalamus, hypothalamus, amygdala and piriform cortex.
Chromatin protein binding was assessed as the fold enrichment in chromatin immunoprecipitation or in DNA adenine methyltransferase identification experiments [ 30, 31].
Non-specific binding was assessed by performing identical reactions in the presence of 1 μM propranolol (Sigma-Aldrich, Saint Louis, MO) as indicated.
