Sentence examples for binding was assessed by from inspiring English sources
The in vitro cell binding was assessed by growing B16F1 cells in culture flasks to 80 90% confluence, trypsinizing, washing in PBS (pH 7.4), aliquoting into 12-well plates and allowing adherence to the wells overnight.
Non-displaceable binding was assessed by adding dextran-coated charcoal (Sigma) admixed with 1% ovalbumin in HEDG buffer to the final concentration 0.15%.
Specificity of the EAAT2 antibody binding was assessed by 5 hours incubation of the undiluted antibody with the immunogenic synthetic peptide corresponding to amino acids 16 31 of rat EAAT2 (Merck Chemicals, Darmstadt, Germany) previous to western blotting (Fig. 2B).
Surface binding was assessed by incubating plasminogen (Glu-plasminogen) with intact log phase cells in the presence or absence of the plasminogen activator tPA and the plasmin inhibitor aprotinin prior to analysis of surface labeling by SDS-PAGE and Western blotting.
Mcd1p binding was assessed by qPCR.
AKT binding was assessed by immunoblot using an HA antibody.
The different recombinant GST-tagged CC2D1A proteins (fragments I, II, III, and VII) were immobilized on glutathione beads and incubated with purified PDE4D5 (IX) and PDE4D5-binding was assessed by western blot.
STAT3 promoter-binding was assessed by the use of a STAT3-specific DNA-binding ELISA (TransAMTM, Active Motiv, via THP Medical Products, Wien, Austria) according to the manufacturer's instructions.
Live cells were spilled from each of the fresh samples and the extent of fluorescent-labelled HPA and Con A-binding was assessed by flowasytometry.
C-SA4503 binding was assessed in C6 monolayers bindinga counting and in anaesthetized rats by microPET scanning.
