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Cell surface fiber binding was assessed at 12, 16, 20 and 24 hr post infection.
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For CB1R and CB2R, non-specific binding was assessed using WIN-55212 at 10 and 5 μM, respectively.
Protein binding was assessed with hyperimmune anti-FN serum at the dilution of 1 : 100 followed by incubation with HRP-conjugated anti-mouse IgG (Sigma) (1 : 500).
Calcofluor binding was assessed following growth of SL1344 and mutant strains at 25 and 37°C on CFA agar supplemented with fluorescent brightener 28.
Intrinsic fluorescence could not be used to assess QacR binding to Dq because Dq displays significant fluorescence at 340 nm, and therefore, Dq binding was assessed by isothermal titration calorimetry.
Stability, log P and Protein binding was assessed.
Plasma protein binding was assessed using ultrafiltration.
LDL-proteoglycan binding was assessed using surface plasmon resonance.
Non-specific binding was assessed with co-incubation with 10 μM PK11195 (Sigma-Aldrich).
In addition, specific binding was assessed using autoradiography on ALK5 expressing MDA-MB-231 tumor sections.
[35S]GTPγS-stimulated binding was assessed with NXT TOPCOUNTER and expressed as a percentage increase in basal [35S]GTPγS-binding.
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