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TSPO binding was assessed as described above in the cortex, corpus callosum, hippocampus and hippocampal subregions (CA1, CA2 and CA3/hilus), thalamus, hypothalamus, amygdala and piriform cortex.
In vitro p21-PCNA binding was assessed as described previously (He et al, 2005).
Chromatin protein binding was assessed as the fold enrichment in chromatin immunoprecipitation or in DNA adenine methyltransferase identification experiments [ 30, 31].
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Statistical significance was assessed as indicated.
Nanoparticle exposure was assessed as follows.
Intracellular staining for GR expression and binding was assessed by FCM as described above.
[35S]GTPγS-stimulated binding was assessed with NXT TOPCOUNTER and expressed as a percentage increase in basal [35S]GTPγS-binding.
Nonspecific binding was assessed using an APC-conjugated rat IgG2b antibody as an isotype control.
The total amount of protein binding was assessed using a scintillation counter and results were recorded as counts per minute and compared as a percentage to binding of wildtype Munc18-1 protein.
Plasma protein binding was assessed using ultrafiltration.
Stability, log P and Protein binding was assessed.
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