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The degree of BldD binding was assayed at a single time point in WT S. venezuelae and the WT overexpressing either the DGC CdgB or the PDE YhjH (the strains whose phenotypes are described above).
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The binding of wild-type and mutant Hfq were assayed at 0, 5, 10, 25, 75, 150, 250, and 500 nM protein over 400 s, in the absence or presence of 1 μM chemically synthesized RydC (Dharmacon, GE Healthcare, UK).
Each KH domain was assayed for kcRNA binding by filter binding assay (Fig. 5).
[ER] status was assayed by standard ligand binding assay.
To further test this idea, one high affinity and one low affinity ligand for anti-ADP3 antibodies (1 and 7, respectively; see Figure 4A) were resynthesized on 160 μm TentaGel beads and approximately 250 of the beads were assayed for binding at different IgY concentrations.
Peptides were assayed for binding affinity using a filtration-based competitive assay to sstr2.
Cells isolated at different stages of the cell cycle were assayed by ChIP for binding of MRE11 and NBS1.
The binding affinities of BubR1 (wild type and variants) for B56γ1 (wild type and variants) were assayed on an iTC200 microcalorimeter (MicroCal) at room temperature.
The binding affinities of butein, 2',4,4'-trihydroxychalcone and fisetin against MtbHadAB were assayed using an ITC-200 microcalorimeter (MicroCal) device at 25°C.
And I guess what I'm assaying is we here at the White House agree".
No binding was observed in the assay.
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