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Peptide binding was analyzed with a 60-second association phase followed by a 60-second dissociation phase.
COL9A1 promoter binding was analyzed with specific PCR primers that recognized only the transiently transfected promoter construct.
SF9 cell purified UL141 was exchanged to Biacore running buffer, TRAIL DR Fc fusion proteins and hLTβR:Fc (negative control) were immobilized on an on an anti-human Fc capture chip, and binding was analyzed with a Biacore 3000 (GE Healthcare) essentially as described (Wang et al., 2010).
Binding was analyzed with BIAevaluation software (Biacore) using a 1 1 binding model.
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Ligand-binding was analyzed with anti-GST antibodies.
To this end, the in vivo p53/DNA binding was analyzed in SKBR3 cells with ChIP assay.
Annexin V binding was analyzed on a FACSCanto flow cytometer equipped with a fluorescence emission at 530 and 575 nm using a fluorescence excitation at 488 nm.
(B ) Jurkat T cells were cultured with inhibitors of glycosylation, then ConA binding was analyzed by flow cytometry.
(B ) Colo205 cells were cultured with inhibitors of glycosylation, then ConA binding was analyzed by flow cytometry.
For the binding assay, cells were incubated with increasing concentrations of Rh-labeled EGF, and binding was analyzed using flow cytometry.
Virus binding was analyzed by flow cytometry.
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