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Cell surface fiber binding was analyzed by flow cytometry at 12, 16, 20 and 24 hr post infection.
Normoxic or hypoxic cells were incubated with C4BP-FITC or prothrombin-FITC both at 0.1 mg/ml and binding was analyzed by flow-cytometry.
Compounds were tested at 1 mM or 2mM each and binding was analyzed by monitoring changes in the 15N HSQC spectra.
Live, late-apoptotic and necrotic cells (as determined by staining with annexin V and Via-probe) were incubated with increasing concentrations of purified C4BP-PS and the binding was analyzed by flow cytometry.
Virus binding was analyzed by flow cytometry.
The binding was analyzed by flow cytometry.
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The specificity of the steroid-binding was analyzed by binding competition using the following steroids: testosterone, dehydroepiandrosterone sulfate (DHEAS), androstenedione, estradiol, and dihydrotestosterone (DHT).
Integrity of transcription factor binding sites was analyzed by comparing the SNP distributions within and outside the putative transcription binding sites.
The biotin binding of the steroid-binding proteins was analyzed by the biotin-coated sensor chip.
DNA binding ability was analyzed by absorption spectroscopy and fluorescence technique using ethidium bromide (EB) as a fluorescent probe.
As such, the binding environment was analyzed by computing the 3D protein ligand interaction fingerprints (TIF) between each drug and the amino acids of the antigen-binding pocket of HLA-B*57 01 HLA-B*57 01 [74
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