Sentence examples for binding was also obtained from inspiring English sources
The TOP10 background strain binds to glycosphingolipids of the ganglio-series, as e.g. fucosyl-gangliotetraosylceramide (Fucα2Galβ3GalNAcβ4Galβ4Glcβ1Cer; No. 8 in Table 1; Fig. 3C, lane 1, lower band) [15], and this binding was also obtained with the recombinant TOP10-CS6 strain (Fig. 3D).
A tree that is more consistent with our binding specificity data was also obtained using the Prank algorithm (Supplementary Figure S6), which uses phylogeny-aware gap placement and scores insertions and deletions more accurately than other alignment programs (Löytynoja and Goldman, 2005, 2008).
Similarly, a specific DNA binding sequence of WIZ was also obtained from software analyses and was confirmed at PARD3, ABCA13, SENP5, and NRXN3 loci.
From the studies of the interaction in solution, the diffusion coefficient of free and DNA-bound NR, binding constant and binding site size of the DNA NR complex was also obtained simultaneously by non-linear fitting analysis of voltammetric data.
Since the haem-access channel was also identified as a possible RB19-binding site, the G169L variant was also obtained, whose leucine side chain completely blocks the haem-access channel, as shown in the crystal structure of a variant including the G169L mutation, compared with the WT DyP (Supplementary Figures S2B and S2A respectively).
Additional validation for the enrichment of MYCN in the antibody precipitated fraction was also obtained through qPCR based analysis of binding sites (Figure S2).
