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Routinely, cyanide binding was also monitored by using the diode array detector (Applied Photophysics), which allowed the synthesis of artificial sets of time-dependent spectra as well as spectral analysis of enzyme intermediates.
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In all analyses, sdAb binding to irrelevant antigens was also monitored to ensure signal was not due to non-specific binding of unfolded sticky protein.
As a negative control, binding of the factors was also monitored at an irrelevant DNA region (negative control [NC] DNA).
The binding of iron protoporphyrin IX was also monitored in the presence of 10 mM Na2S2O4 to reduce the iron III) protoporphyrin IX to the Fe(II) species (Fig. 1B).
Blood glucose was also monitored.
Efficacy was also monitored.
Body weight was also monitored.
Mortality was also monitored daily.
Call length was also monitored.
Safety was also monitored by an independent data monitoring committee.
Body-weight changes was also monitored.
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