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Specificity of binding was also determined using wild-type and mutated consensus oligonucleotides.
Receptor binding was also determined by autoradiography in vitro.
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Beside the binding capacity for the IgG standard, the binding capacity was also determined for IgG in human plasma.
As a control for plausibility the copy number of mRNA for the low-abundance housekeeping gene TBP (TATA-box binding protein) was also determined and was expected to be between 20 and 40 copies per normal resting cell (data not shown).
The content of metallothionein, a cysteine-rich zinc-binding protein, was also determined in the tissue.
DNA-binding activity of CREB was also determined by colorimetric assay using the TransAM ELISA kit.
The binding of Achatinin-H with PA was also determined using anti-Achatinin-H antibodies by ELISA [3].
The binding affinity of sdAbA1 for CypA was also determined by surface plasmon resonance, yielding a ka of 5.67 × 10/M/second, a kd of 3.91 × 10 3/second and a calculated KD of 6.9 × 10 9 M.
The effect of the mutation of the P-TEFb binding or RNA binding domains of Tat on P-TEFb release was also determined.
The amount of nonspecific binding of 11C-TMSX to the filter was also determined by applying the same procedure to a sample created by addition of 11C-TMSX to saline.
To more accurately reflect the situation in normal brain, the binding of these peptides to adult neurons was also determined in a mouse brain slice model.
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