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This cell surface binding was also demonstrated dose dependently by flow cytometry.
A role for PE in PA-effector binding was also demonstrated by Young et al. [ 28] for binding of a transcriptional repressor in yeast, Opi1, which will be discussed in more detail in the next section.
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The binding is also demonstrated to shift the monomer-fibril equilibrium in favor of dissociation.
Weak binding affinity was also demonstrated for P6CON, a high affinity binding site for Pax6 [52], the human homolog of Eyeless (Fig. 5B, compare lanes 1 and 5, and Fig. 5C, left panel for quantitative comparison).
In addition to this [H]-VUF11211 based profiling, for proof of principle, the ability of metabolites to displace the natural orthosterically binding chemokine was also demonstrated using a I-CXCL10-based radioligand binding assay.
Detection of single biomolecular binding events was also demonstrated using nanoparticles as labels.
The impact of LL-37 binding to polysaccharides was also demonstrated in an animal model of Pseudomonas sinusitis biofilm, where a high concentration of LL-37 derived peptide was shown to eradicate Pseudomonas biofilms and decrease bacterial viability [21].
A potent role for the active form of lovastatin in increasing trastuzumab binding to cancer cells was also demonstrated via competitive radioligand saturation-binding assays (Fig. 2h and Supplementary Fig. 4c).
Specificity of binding to GLP-1R was also demonstrated.
125I-labeled calmodulin binding to Ca/calmodulin protein kinase was also demonstrated using filters containing Ca/calmodulin protein kinase transferred from polyacrylamide gels after two-dimensional gel electrophoresis.
Sulfation of CDR tyrosine residues, a property recently shown to broaden antibody binding affinity and antigen recognition was also demonstrated.
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