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When all basic amino acids at positions 7, 9 and 10 were replaced by Ala (RMS-P3/AA), binding was almost completely abolished (70-fold decrease).
Whereas C1q and factor H were only partially blocked by mAb CS-56 and may likely have additional sites on platelets, the observation that C4BP binding was almost completely inhibited by this antibody suggests that CS-A is the primary interaction site for C4BP on activated platelets.
In addition, CC2D1A-PDE4D5 binding was almost completely abolished in the absence of the first DM14 domain (fragment II).
In both E210A and K141E, cyanide binding was almost completely impaired, and in the latter variant the reduction potential was more positive than in the native enzyme.
Binding was almost completely restored by treating the infected cultures with the trypanocidal drug nifurtimox [ 52].
Similar(53)
Significantly however, Neu5Ac-binding was almost completely abolished when C. jejuni 11168-O was grown at 37°C and 42°C (Figure 4B).
At WP1066 concentrations of 10 μM, the promoter-binding of STAT3 was almost completely prevented (Fig. 3a).
Importantly, with enforced expression of HB-MSH2Y238F, the binding of MSH2 to MSH6 was almost completely restored (lane 4).
As depicted in Fig. 4A, when 2 mM ACA was added to the reaction, the binding of the proteins to PLG was almost completely abolished.
While the DNA binding activity in extracts isolated from Id1-expressing cells was almost completely abolished, the level of E-box binding complexes in Hes1 transgenic thymocytes was comparable to that detected in wild type cells.
It was found that the binding of HeV G mutants D260A, G439A, K443A and K465A to both m101 and m102.4 was almost completely eliminated, while the binding activity of mutants G449A and D468A was significantly decreased.
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