Sentence examples for binding was allowed to from inspiring English sources

The binding was allowed to proceed at 4°C overnight.

In contrast to the previous experiment, this binding was allowed to saturate, and after ∼30 min a small but constant elongation rate was observed.

After binding was allowed to proceed for 12 hr at 4°C, the resin was washed and captured protein was eluted as described above.

Specific antibody was added to the cleared lysate and the binding was allowed to proceed at 4°C overnight before Staphylococcus aureus Protein A (Sigma, #P7155) was added to capture the immune complex and then washed extensively.

Binding was allowed to continue over time until convergence.

Binding was allowed to proceed for 1 h at room temperature with shaking at 900 r.p.m.

Competitive binding reaction was allowed to proceed and the signals were generated as described by Darwish et al.[32].

Binding reaction was allowed to proceed and the signals were generated as described in the Experimental Section.

The binding buffer was allowed to flow over the sensor chip for 15 min at a rate of 30 μL/min to allow for the dissociation of bound MAb/scFv from the antigen on the chip.

For supershifts, 1 µl of anti-p65 antibody (Rockland) or 2 µl of anti-p50 antibody (Santa Cruz, SC-7178) was added and the binding reaction was allowed to proceed for an additional 15 min. Protein−DNA complexes were resolved on a non-denaturing polyacrylamide gel and visualized by autoradiography.

The virus antibody binding reaction was allowed to take place during an hour incubation at 37°C.