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Calorimetric studies revealed that the binding was accompanied by a large positive entropy term with a small negative enthalpy contributions.
Through use of the environment-sensitive fluorophores acrylodan and Nile Red, it was observed that the activation of peptide binding was accompanied by enhanced peptide and solvent accessibility to a hydrophobic binding site(s).
Combined NMR and MD analysis of PaDDAH showed that ligand binding was accompanied by a disorder-to-order transition for the L1 loop.
Western blot analysis revealed that loss of clathrin and AP-2 binding was accompanied by the loss of CI-M6PR, EpsinR and PI3KcIIα in the anti-GFP-OCRLX3 immunoprecipitates.
In addition, quantitative RT-qPCR experiments demonstrated that this in vivo binding was accompanied by a significant induction of Hsp70 and Hsp90 transcription (1.78 and 1.70 fold, respectively; P < 0.005; n = 8; Fig 1D).
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In addition, MITF and c-FOS also bind to regions not bound in HPC7, and this "new" binding is accompanied by relocation of shared TFs such as GATA2 to these regions (see arrowhead in Fig 5A, right panel).
TCR phosphorylation in response to pMHC binding is accompanied by segregation of the transmembrane phosphatase CD45 away from TCR-pMHC complexes.
This zinc binding is accompanied by a large shift in the backbone conformation of the 99-loop and by large movements of both His side-chains.
Inhibitor binding is accompanied by a structuring of the region comprising residues Tyr71 Gly80 from being mostly disordered in the apoenzyme and leading to positioning of crucial active site residues for establishing enzyme inhibitor contacts.
The conventional thin section TEM thus confirmed that cell surface binding is accompanied by size changes of the core, but otherwise failed to reveal all other changes of the core seen by cryo-ET.
GTP binding is accompanied by conformational changes in both RF3 and the ribosome (Gao et al., 2007).
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