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However, GST-E2F1 binding was abrogated when both sites were absent, consistent with the notion that tandem bona fide E2F recognition elements in the EBP1 promoter are centered at positions −158 and −113, relative to the transcription start site.
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Colony forming ability and leukemogenicity of a fusion protein (MLL-AF9) was abrogated when the MLL-derived segment was replaced with the DNA binding domain of CXXC1 [ 27].
However, ATF4 stimulation was abrogated when a 132-bp region between bp −215 to −83 was deleted (Fig. 7C).
As with earlier analyses this signal was abrogated when the expression profile was 'randomised'randomised
Importantly, detection of CHD4 phosphorylation was abrogated when Ser-1346 was mutated to Ala (SA; Figure 3A).
This function was abrogated when three out of the four lysine residues were substituted to alanines.
Notch1 increase was abrogated when PKA activation or Notch receptor cleavage were inhibited.
The CMG formation was abrogated when the N-terminus of Cdc45 is removed.
The Akt-1-mediated effect was abrogated when the N-TEV-BadS136A mutant construct was used (Fig. 7f,l).
Strikingly, this protective effect was abrogated when rVV-NP challenge was delayed until 6 months after influenza infection (Fig. 2).
For example, the spontaneous onset of T1DM in nonobese diabetic mice was abrogated when treated with recombinant GAD65.
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