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4) The experiments establishing ATP binding use MANT-ATP and MANT-ADP.
Capillary leak, fluid loading, decreased protein binding, use of vasoactive medications and altered excretory organ function, will significantly distort the 'normal' pharmacokinetic (PK) profile of many agents [ 2].
Most published analyses of H-IP3 binding use media similar to TEM because its high pH and/or low ionic strength reduce the Kd of IP3R for IP3, thereby increasing the specific binding determined with low concentrations of H-IP3.
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Therefore, we produced four different cPPR proteins designed to bind homopolymers of adenine, cytosine, guanine or uracil and tested their binding using ssDNA homopolymers (Fig. 1d).
An example of imaging GDI Cdc42 binding using the wild-type GDI.Cdc42 FLARE biosensor.
A zoomed example of imaging GDI Cdc42 binding using the wild-type GDI.Cdc42 FLARE biosensor.
RBFOX1/2 regulate hundreds of splicing events as measured by their direct RNA binding using RNA CLIP and RNA-seq experiments20,21,22.
Sandanaraj, B. S., Demont, R., Aathimanikandan, S. V., Savariar, E. N. & Thayumanavan, S. Selective sensing of metalloproteins from nonselective binding using a fluorogenic amphiphilic polymer.
Chromatin silencing was examined by measuring H2AK119ub, and by Bmi1-specific binding using hearts under oxidative stress (Bmi1+/−) and with reverted phenotype (Bmi1+/−G6PDtg) (Supplementary Figure 5E).
Alakurtti K, Aalto S, Johansson JJ, Nagren K, Tuokkola T, Oikonen V et al. Reproducibility of striatal and thalamic dopamine D2 receptor binding using [11C]raclopride with high-resolution positron emission tomography.
Here, fluorescence-based detection provided sensitive detection of binding using minimal amounts of materials.
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