If it can be determined that GL3-GL1 does not in fact dimerize or bind to two separate binding sites in the CPC promoter, and that it is in fact a stable complex, then the current view of the core trichome differentiation network (Fig. 1) must be incomplete.
Megalin binds to two separate sites on RAP [ 66] with high affinity, and the binding is calcium-dependent.
Monoclonal antibodies 18H4 and 19F12 against alpha-foetoprotein (AFP) were examined by enzyme immunoassay for binding to two forms of AFP that were separated on the basis of the reactivity with lentil lectin (LCA).
Using chromatin immunoprecipitation, we determined that this Zorro LNA altered CTCF and RNA polymerase II binding at three separate and distinct regions in the NF1 gene.
Receptor-activation studies can be performed comparing binding from two separate scans, i.e. one at baseline and one activation/intervention study; this has been the most frequently reported approach.
Rad52 has greater binding affinity for ssDNA than dsDNA (46, 47) and appears to have two separate binding sites for each in the N-terminus of Rad52 (48).
The KD of the interaction was calculated to be 7.3 µM±1.5 µM by analysis of the equilibrium binding data from two separate experiments (shown in Fig. 4B) using the Langmuir binding model.
In the case of the neural exon of the Src gene, which is repressed in most tissues by the binding of PTB, two separate pyrimidine tracts on either side of the exon cooperate to form an ATP-resistant complex containing unknown numbers of proteins (Chou et al, 2000).
As the myosin-binding mutants did not exert this effect, substrate binding and chain formation seem to be two separate activities of UNC-45.
Interestingly, the Oct-4B isoform does not bind to a probe carrying the Oct-4 consensus-binding sequence, and two separate regions of its NTD are responsible for inhibiting DNA binding (Lee et al, 2006).
