Sentence examples for binding to this site from inspiring English sources

Here we demonstrate the engineering of a Zn2+ binding site at the predicted dimeric interface of the dopamine transporter (DAT) corresponding to the external end of transmembrane segment 6. Upon binding to this site, which involves a histidine inserted in position 310 (V310H) and the endogenous Cys306 within the same DAT molecule, Zn2+ potently inhibits [3H]dopamine uptake.

Traditional drug design approaches involve tightly binding to this site and blocking its activity, under the assumption that the target protein exists in a single rigid structure.

Upon binding to this site, bioflavonoid can directly interact with hematin of the COX enzyme and facilitate the electron transfer from bioflavonoid to hematin.

Correspondingly, no Ca2+ binding to this site of the C2B domain was observed in the recently determined crystal structure of a synaptotagmin-3 C2AB fragment [47] (Figure 5D).

To monitor structural changes elicited by inhibitor binding to this site, we measured fluorescence spectra of oxaloacetate decarboxylase and of its subunits in the absence or presence of the inhibitor.

Fluorescence Correlation Spectroscopy (FCS) affinity assays indicated that CaM binding to this site is of lower affinity as compared to the other sites [18], raising doubts on whether it actually binds CaM or merely contributes to CaM binding to BD-C2.

Further work to identify the DNA-binding protein that binds to this site will help to elucidate the mechanistic basis of how variants within the MLH1 5′UTR affect expression.

Inhibitor binding to this allosteric site changes the conformation (i.e., tertiary structure or three-dimensional shape) of the enzyme so that the affinity of the substrate for the active site is reduced.

As noted above, there is no evidence in our structures for binding to this second site.

Because microRNA binding sites are important for mRNA translation and degradation, the variant T allele of rs2016347 might disturb binding to this microRNA site [ 34].

Interestingly however, caveolin-3 binding to this same site via a WW-like domain [ 36] appeared insensitive to the tyrosine phosphorylation [ 28].