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Our time-resolved single-molecule analysis reveals the underlying heterogeneity of the action of GroES/EL on a bound polypeptide substrate, which might arise from the random nature of the specific binding to the various identical subunits of GroEL, and might help explain why multiple rounds of binding and hydrolysis are required for some chaperonin substrates.
Site-directed and random mutagenesis as well as domain-swapping, NMR and X-ray cristallography have made it possible to get detailed insights in the molecular mechanisms that govern IgG/FcγR interactions and to define some of the structural determinants that impact IgG binding to the various FcγR.
Based on prediction models for peptide binding to the various MHC class I alleles of these 7 subjects, there were over 200 potential new Gag epitopes identified within the 80 positive Gag 15mer peptides (data not shown).
[15] The investigation of ligand binding to the various iGluR subtypes is in the focus of ongoing research.
Since the viruses used in our study only differ in the gp120 V3 loop region, the enhanced binding to the various CXCR4-g1 mutants can be interpreted as an effect of the mutated coreceptors and the V3 loop.
Flow cytometric analysis of human P- or L-selectin/μ binding to the various CHO-PSGL-1 transfectants showed that P- and L-selectin/μ bind similarly to human, bovine, pig, rat or equine PSGL-1 expressed by transfected CHO cells.
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Because ELISA cannot distinguish between monomeric and multimeric forms of parvalbumin, this technique is not suitable for attributing IgE-binding to the various forms of our parvalbumin as found in our glycosylated samples.
These pharmacophores explain the selectivity of binding to the MATs for various compound classes and have been used to search in silico databases for novel, potentially selective ligands.
Under these conditions, Mig1p is predominantly in the dephosphorylated state and translocates into the nucleus [ 17, 19] to repress genes by binding to the URS of various genes.
It binds AT-rich sequences, and previous reports have demonstrated HMGA1a binding to the authentic promoters of various genes.
We assayed ArntbHLH-C/EBP and ArntbHLH for binding to the E-box in various buffers, for we found that protein:DNA binding activity absolutely depended on conditions of experimentation (see Materials and Methods for details).
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binding to the active
binding to the distal
binding to the N-domain
binding to the anti-BL
binding to the mammalian
binding to the bacterial
binding to the AMP-lid
binding to the imprinted
binding to the degenerate
binding to the Smad
binding to the nucleobase
binding to the urinary
binding to the different
applicable to the various
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