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The reaction mixture was then transferred to the insert cup of Ultrafree-MC centrifugal filter unit (MW cut-off, 5 kDa), which had been prewashed with 400 μL 1% bovine serum albumin,1% sucrose solution to minimize proteasome binding to the unit, and the mixture centrifuged at 7,000 rpm for 1.5 hr at 4°C, which resulted in about 80 μL of the proteasome preparation in the insert cup.
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As expected, the C-terminal domain of RNA polymerase II antibody did not bind to any of the AMP gene promoters but showed weak binding to the Act5C transcription unit.
29 FoxO1 suppresses AR transactivation by binding to the transcription activation unit 5 (TAU5) located in the AR N-terminal domain (NTD).
The chains are connected into a 3D CdSO4-type array via the second Hg CN 2 unit binding to the chlorides; adjacent chains are inclined at 59.0°.
The thermal stability of the LysM domain was slightly increased upon binding to NAG units (Ohnuma et al. 2008).
114, 277– 296, 296– 298 Hg2+ ion sensing occurs due to binding to aptamer units crosslinked with PAAm which results in shrinkage of the hydrogel-colloidal PhC.
A similar reasoning holds for the binding of B to the functional unit.
Thus, it appears that two factors play a role in the dramatic sensitivity of sensor 6: (i) as in all the other sensors synthesised, binding of Zn2+ to the ligand unit deactivates the PET quenching process leading to a "switch on"; (ii) the aggregation of the unbound sensor initially serves to quench the emission but binding of Zn2+ changes the aggregation such that fluorescence is recovered.
It acts similarly to tulathromycin by irreversibly binding to receptors of the 50S ribosomal units [ 29].
This might support the notion that the binding of cardiotonic drugs to the regulatory unit of cardiac troponin occurs in a similar mode.
The p Ka of His86 decreased 0.61 pH unit upon binding to CMG2 (from ∼6.7 to 6.1), while the p Ka of His310 increased 1.68 pH units.
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