Sentence examples for binding to the template from inspiring English sources

The lengths of the primers with the preferred mutation site were designed between 25 to 45 bases; this number of bases is required for specific binding to the template DNA.

Of note, the addition of hNAP1 and SET to the binding reactions reduced H1 binding to the template (Fig.  4a, b, lane 2).

Processivity is defined as the number of nucleotides added by a DNA polymerase per one binding to the template DNA, and in general, replicative DNA polymerase has a high processivity value, and repair polymerase has a low processivity value.

It is reasonable to assume that a prolonged polymerization time increases the diameter of particles which are less efficient in binding to the immobilized template due to sterical factors.

In general, we found that chaperone binding to the immobilized template varies between experiments, but is generally low or undetectable.

Specificity was demonstrated by the ability of cold specific competitor but not the non-specific competitor to prevent SmTRα binding to the oligonucleotide template.

Translation initiation occurs with a ribosome binding to the mRNA template and begins at the start codon with incorporation of the first amino acid of the polypeptide.

RNA processing factors can be recruited to transcribed genes in multiple, not mutually exclusive, ways: i) by binding to the DNA template either directly or through recruiting factors, ii) by binding to processing signals present in the nascent RNA and iii) by binding to defined regions of the RNAPII elongating complex i.e. CTD [ 60].

Third, KiNRTIs values increased sharply with NRTI-resistant RT (ERT-mt6 RT) at ATP concentrations around the KiATP value (2.8±1.3 mM) for ATP binding to the RT-template-primer complex (Figures 2C and 2D).

We found no marked increases in the Ki values of AZTTP and d4TTP at the ATP concentrations around the Ki' ATP value (1.1±0.4 mM) for ATP binding to the RT-template-primer-dTTP complex for the excision reaction (Figures 2C and 2D).

This conserved His residue within the His-containing loop is necessary for proper binding to the nucleic acid template [ 33].