Resultant radioactive metabolites could also contribute to nonspecific binding or compete with the parental tracer for binding to the target.
Positron emission tomography, or PET, can be used to tell if a drug is binding to the target protein in the body.
Likewise, bivalent binding to the target may negatively influence the therapeutic efficacy of an antibody.
Most of the drug molecules exhibit their biological activity through binding to the target protein.
Through the screening process, a molecule may show potential as a drug by binding to the target biomolecule (i.e., the key for the lock) or killing diseased cells.
Because an α,β-unsaturated moiety can bind to a molecular target covalently, these compounds might induce a Ca2+ influx via covalent binding to the target.
The addition of strept-AuNPs, binding to the target due to the strong streptavidin biotin interaction, led to a further increment of Rct thus obtaining significant signal amplification.
ADCs are prodrugs that are inactive in circulation but exert their cytotoxicity upon binding to the target cancer cell.
To improve access and binding to the target we have now designed novel N10,N11-di-alkylamine N10,N11-di-alkylamine N10,N11-di-alkylamine
The use of CETSA HT rather than an alternative assay measuring a functional response can therefore increase the confidence that the test compound is directly binding to the target protein.
Aptamer pools from each round of selection were tested for their binding to the target and non-target cells using flow cytometry.
