Sentence examples for binding to the probe from inspiring English sources

At 20X excess, the wild-type oligonucleotide prevents CREB binding to the probe immobilized on the plate.

Two bands were evident upon incubation of the wildtype probe with the d16.5 pregnant mammary gland nuclear extract (Figure 6b, lane 1) indicating binding to the probe.

We postulate first that hemoglobin may compete with the target mRNA in binding to the probe, thus contributing to a higher-than-normal pseudo binding background signal.

Truncation of the entire proline-rich N-terminus region until amino acid 181, giving rise to FOXP3-ΔN, was required for strongest binding to the probe (Figure 5B, lanes 6, 7).

Further, analysis by EMSA of the binding of ISGF3 (a complex of STAT1, STAT2 and IRF9) to a DNA probe containing the ISRE of the ISG15 promoter indicated that IFN-α-induced binding to the probe was equivalent within extracts of EBV-negative (A.2) and EBV-positive (A.15) Akata cells for at least 4 h following addition of IFN-α.

Consistent with the EMSA result, Western blot analysis with STAT5 antibody confirmed that STAT5 was the transcription factor binding to the probe S1 in the complex (Fig 3D).

Our k-mer based PBM model is motivated by the biophysics of TF binding to the probes in PBM experiments.

This method was useful but also had problems related to specificity and sensitivity of the fragments of DNA binding to the probes which are difficult to control.

Conventional dot-blot hybridizations need to be incubated for several hours in plastic bags or glass tubes and require large volumes to cover the whole membrane, which decreases the concentration of DNA available for binding to the probes, also decreasing active interactions between complementary strands considerably [ 33].

However, p53 with mini deletion in MCF7/AdrR cells, which lost its DNA-binding capability, did not bind to the probe.

Incubation with a 100-fold molar excess of the unlabelled mutated probe did not alter binding to the wildtype probe (lane 3) but addition of unlabelled wildtype probe competed off all binding (lane 4).