Sentence examples for binding to the plates from inspiring English sources

Serum IgG or IgE binding to the plates was measured with HRP-conjugated anti-mouse IgG or IgE, respectively.

ACPA binding to the plates was confirmed by standard ELISA in separate coated wells (data not shown).

Recombinant galectin-1 protein (see [ 20] and Supplemental data for method of preparation and Supplementary Fig. 1 for purity analysis) was pre-incubated with serial dilutions of TDG or sucrose (as a control disaccharide not binding the galectin-1 CRD) at 37°C for 1 h before testing for protein binding to the plates of laminin or cells.

An hour incubation with a second antibody conjugated to horseradish peroxidase (HRP) and developing chemiluminescent reagents were used to detect activated Ras binding to the plate.

We opted for plating the cells at ∼80% confluence at the time of experimentation, in defined media void of serum and in optical black-sided well plates, all of which resulted in the least non-specific binding to the plate, reduced light scattering and gave the greatest percent of specific binding.

Antibodies directed against AP-1 family members used in this study were described above, an antibody directed against RNA polymerase II (sc-899) was also used, and a purified IgG I-10000, Vector Laboratories) was used as a control for immunoprecipitation to test aspecific binding to the plate.

VEGF-A binding to the plate is then detected using a polyclonal anti-VEGF-A-HRP conjugate.

The denatured Hb or HbA1c was neutralized by the addition of more than ten times the initial volume of 0.1 M sodium carbonate buffer (pH 9.5) for the efficient binding to the ELISA plate.

At the highest tested concentrations (c. 0.1 1 mM PEG), both competitors blocked the binding to the assay plate completely.

SigA incubated in tissue culture wells in the absence of HEp-2 cells (Fig. 1, lane 2) was included as a control for non-specific binding to the tissue culture plate.

Addition of Cit573 reverted up to 80%% of ACPA binding to the CCP2 ELISA plate (data not shown).