Sentence examples for binding to the origin from inspiring English sources

Of interest, Orc1 binding to the origin was not detectable in G0, despite the protein being expressed; in early G1, the protein started to associate with the origin and its enrichment peaked in mid G1 (4–5 fold).

On this basis, we propose that the primary determinant of E2F binding to the origin regions might not be primary DNA sequence recognition by E2F itself, but its interaction with chromatin-bound Rb, consistent with the information that chromatin-bound Rb is also detected during the S-phase [36].

In contrast, E2F1 showed reciprocal behavior, namely commencing association with the origin as soon as it was expressed (5 h; ∼2-fold enrichment over background), while its binding to the origin increased at 10 and 15 h (G1/S and S respectively; ∼4 5 fold enrichment).

The relaxase initiates conjugation by binding to the origin of transfer (oriT), a process which is facilitated by MobC.

DnaA-ATP binding to the origin must determine the timing of initiation for a range of growth rates and thus in the presence of increasing genome amounts (providing non-specific binding sites).

Both deletion mutants were inserted into the VPS13 locus and Cdc45 binding to the origins was monitored throughout S phase.

To follow the firing of origins, cells were arrested in the late G1 phase with α-factor, released synchronously into S phase (Fig 1A) and Cdc45 binding to the origins was determined by chromatin immunoprecipitation (ChIP) assay.

To further validate the idea that recruitment of Cdc45 is tightly coupled to initiation of DNA synthesis, we confirmed that its binding to the origins occurs simultaneously with that of Pol2, the catalytic subunit of replicative DNA polymerase ɛ (Fig 1B H).

The AT-hook motif has been shown to be required for the replication and partitioning of Epstein-Barr virus oriP plasmids [ 30], as well as for the origin recognition complex binding to the replication origin in fission yeast [ 31].

The level of Cdc45 binding to the rDNA origin of replication (rARS) in the eco1 mutant peaked at 90 min, earlier than the peak at 105 min observed in WT cells (Fig 3A), further confirming that the rARS fires earlier in the eco1 mutant than in WT.

Given these considerations, our first speculation has been that binding Rb/E2F to the origin region might be mediated through the interaction of either protein with known components of the pre-RC.