Sentence examples for binding to the operator from inspiring English sources
Both of them inhibit the LacI binding to the operator DNA without affecting its binding to non-operator sequence.
TetR2 protein represses the system by binding to the operator sites of the tet operon.
Allolactose is the inducer of the lac operon, binding to the lac repressor and inducing a conformational change that prevents the repressor from binding to the operator region.
Both lac system and tet system use a repressor binding to the operator lacO/ tetO in the absence of specific inducers, and the addition of the inducer can activate downstream gene expression [ 34].
We created a new DNA recognition specificity for tetracycline repressor (TetR) binding to the tet operator variant tetO-4C5G containing four bp exchanges compared to tetO.
The inducer weakens the protein-DNA binding, rendering the mutant capable of freeing itself from non-specific DNA binding to the lac operator [48].
However, the use of tag technology may be problematic since, in experiments aimed at describing RcsB binding to the flhDC operator sequence, others reported that RcsB binding to DNA was not detected unless the His-tagged RcsB concentration in the reaction mixture reached ≥1.5 µM [21].
As an effector for GlpR, G3P can bind to the effector-binding domains of GlpR, and then will prevent the HTH DNA-binding domains of GlpR from binding to the DNA operator sequence of glp regulon [ 24, 25, 27].
In order to assess the specificity of AtoC binding to the predicted operators, we re-examined all ChIP analyses by reducing the PCR cycles to twenty five, while ChIP signals for both acetoacetate-induced and non-induced cultures were analysed comparatively for each target.
Possibly, this site, referred to as Box6, is an auxiliary operator site that supports binding to the main high-affinity operator sites, besides the intragenic Box4 and Box5, which were identified previously as secondary operator sites (Additional file 9: Figure S6) [ 35].
