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The system was constructed as two layers, spatially presenting the chondroinductive transforming growth factor-β1 (TGF-β1) in one layer and the osteoinductive bone morphogenetic protein-4 (BMP-4) in a second layer, via affinity binding to the matrix.
SPR data were corrected for background binding to the matrix of the chip ("blank" channel) and analyzed with BIAevaluation 4.1 software (Biacore, Piscataway, NJ).
This greatly reduced the binding of native E. coli proteins binding to the matrix.
To this end, we evaluated peptide binding to the matrix relative to controls by direct incubation onto the target.
Cytochrome c oxidase (COX) is allosterically inhibited by ATP/ADP (binding to the matrix domain of subunit IV).
The data shown here (Figs 1, 2; supplementary material Figs S1 3) demonstrate that BMP-2 retention by covalent binding to the matrix is essential for the induction of osteoblast precursor differentiation into mature osteoblasts.
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LPPs exhibited enhanced binding to the matrices used in the study, which significantly improved sensitivity and consistency of the assays.
SPARC is a matricellular protein that modulates cell adhesion and growth and modulates cell-matrix interactions by binding to the extracellular matrix [17].
Alternately, GlSMAR58 and GlSMAR16 showed binding to the nuclear matrix by southwestern though it was not amplified from the matrix dependent pool in the PCR based assay.
Collagen type 1α1 is produced from the MEF and represents 90% of total collagen in most connective tissues; integrin α5 is responsible for binding macromolecules to the matrix and is involved in angiogenesis; laminin α1 is the glycoprotein responsible for the binding to the extracellular matrix; cadherins are transmembrane proteins that mediate cell-cell adhesion in animals.
The Sp domain has previously been suggested to bind to glycosaminoglycans, as it promotes aggrecan hydrolysis [ 10] and binding to the extracellular matrix [ 10].
More suggestions(19)
binding to the array
binding to the template
bonding to the matrix
binding to the parent
binding to the arrays
binding to the tyrosine
binding to the FasR
binding to the A-site
binding to the soil
binding to the promoter
binding to the receptor
binding to the β-globin
binding to the target
binding to the fingerprint
binding to the cue
binding to the colchicine
binding to the waaA
binding to the junction
binding to the lipid
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