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(i) MCT2 binds preferentially to embigin over endogenous basigin, with the binding to the latter requiring an interaction between the C-termini of both proteins that is not required by MCT1.
While the majority of crosslinked liposomes did not present lectin binding outside of interfaces, especially vesicles at the border of crosslinked clusters did show an increase of binding to the latter (Fig. 1b, white arrows).
This would be in consonance with reports that show that TIM interacts with actin, microtubules and membranes [27] [30] and that TIM binding to the latter structures is affected in human TIM deficiencies [29].
Although this secondary antibody was raised in the same species as the capture one (goat in variant 1), it revealed some non-specific binding to the latter (data not shown).
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The former mutation destabilizes the binding to the RNAP, while the latter variant is not impaired in RNAP binding, which suggests that the TFEβ WH domain plays a role for the stabilisation of the PIC.
The mAb-competitor mixtures were then added to wells with plastic-adsorbed Candida β-glucan (GG-Zym) and residual mAb binding to this latter was measured as described above.
We reasoned that a combination of NS9283 and sazetidine-A would activate A3B2 receptors, with the former binding to the α interface and the latter to the α interfaces.
The latter mediates indirect binding to the PABPC1 N-terminal domain.
In IP3R, the N-terminus forms the IP3-binding site, which can be divided into the ligand binding 'core' (residues 224 579) and the 'suppressor domain' (residues 1 223), with the latter inhibiting IP3 binding to the core [ 13, 14].
Syntaxin-immunoreactive bands were pulled down from PC12 cell lysates by Kv2.1-C1 and Kv2.1-C1a peptides (Fig. 1B), but not by the Kv2.1-N or Kv2.1 C2 peptides (the binding of syntaxin to the latter was very weak, if any).
At unattached kinetochores, Mad1-bound Mad2 dimerizes with soluble Mad2 to induce binding of the latter to Cdc20 [ 1, 3], an essential co-activator of the anaphase-promoting complex/cyclosome (APC/C) [ 4].
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com