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The fluorescence of these tryptophan residues could change upon ligand binding to the isolated C-terminal region of EAG1 channel, as observed in HCN2 channels with cyclic nucleotides.
We therefore compared IP3 and AdA binding to the isolated NT, initially in TEM.
All mutations that reduce glycogen binding to the isolated GBD also abolished inhibition by bovine liver glycogen.
To allow more direct comparisons with functional assays, we compared IP3 and AdA binding to the isolated IBC and NT in CLM.
Observation of [H]Ry binding to the isolated sarcoplasmic reticulum preparation was performed in the presence of 1 nM [H]Ry and 10 μM Ca2+.
We first measured binding to the isolated sites BS1 and BS2 separately and subsequently global fitting of direct and reverse titrations of the tandem site (62).
Similar(47)
In our structure, the MyTH4 domain has no direct contacts with the DCC peptide, in spite of the yeast two-hybrid assay which demonstrated full DCC-binding affinity to the complete MyTH4 FERM cassette but weaker binding affinity to the isolated FERM domain (Zhu et al, 2007).
The analogues prepared exhibit interesting binding data to the isolated receptors αvβ3 and αvβ5.
The binding of methadone to the isolated AGP preparations was investigated using intrinsic fluorescence studies at the microtitre level.
Consistently, Smac partially impaired the binding of RIPK2 to the isolated XIAP BIR2 domain (Fig 5F, compare lanes 1 and 2).
It is conceivable that this phenomenon is due to binding of truncated toxin to the isolated CROP domain immobilized at the cell surface.
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