Sentence examples for binding to the filter from inspiring English sources
To study the possibility of irreversible binding to the filter, the recovery efficiency of cells from the filters was determined using plate counts and phase contrast microscopy.
The mean percentage of plasma free fraction (ff) and percentage of unspecific binding to the filter matrix of the Centrifuge vials was determined.
The cause of this nonspecific binding to the filter remains unknown.
Non-specific binding to the filter was previously measured and subtracted from the total uptake.
(7) Depending upon the extent of annealing, the concentration of remaining ssDNA may be low enough to result in a significant fraction binding to the filter membrane.
No protein controls (replacement of MVM vesicle protein by IVB) were included in parallel to determine tracer binding to the filter, which was subtracted from total vesicle count.
Samples were incubated for 90 min. Incubation was stopped by rapid vacuum filtration over Whatman GF/C glass microfibre filters (VWR International SAS, Strasbourg, France) presoaked with polyethylenimine 0.3% in order to reduce non-specific binding to the filters.
Heart samples were incubated for 90 min. Incubation was stopped by rapid vacuum filtration over Whatman GF/C glass microfibre filters (VWR International SAS, Strasbourg, France) presoaked with polyethylenimine 0.3% in order to reduce non-specific binding to the filters.
Radiolabeled PEG 5 -BP-USPIOs can be further PEG 5 -BP-USPIOscentrated to the desired volume using a centrifugal size-exclusion filter with a molecular weight cutoff of 10 kDa (note that around 9% nonsPEG 5 -BP-USPIOsto the filters was found).
The data was iteratively fit (through nonlinear regression) using the computer program Deltagraph 4.0 (Red Rock Software) to the equation y = b+c((x/(x+Kd app)), where y is the experimentally measured value (top counts-bottom counts/top counts), x is the protein concentration, b represents nonspecific binding to the protein and/or filter, and c is the maximum fraction of counts that can be bound.
This observation confirmed the fact that relevant inflammation mediators were not eliminated by binding to the plasma separation filter.
