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Exact(23)
Nonetheless, probes were designed to bind at the same positions, and any mismatch decreases binding to the corresponding probe, thereby reducing the resulting netMFI to levels lower than those for perfect hybridization in a situation of competitive dynamics.
Similarly, we observed that the glycoside NIT70A, with the 1-4-linked D3-like extension, blocked PGT125 and PGT126 binding (Fig. 3b), even though no binding to the corresponding glycoconjugate (NIT83A), with on average 3.4 ligands/mol BSA, was observed.
Altogether, the ensembles suggest that, upon initial substrate binding to the corresponding lid site, the opposing lid is maintained open and accessible for subsequent substrate binding.
The potential utility of designed bivalent ligands as targeting agents for cancer diagnosis and/or therapy can be evaluated by determining their binding to the corresponding receptors.
Although detection strategies are different for various aptamer-based assays, the core of the design strategies is similar towards reporting the presence of specific target binding to the corresponding aptamers.
The biofunctionality of these plasmonic nanostructures has been demonstrated by fluorescent microarray immunoassay and LSPR measurements, recording the shift of the RP produced after the antibody binding to the corresponding hapten-oligonucleotide probes immobilized on the nanostructured surface.
Similar(37)
Having shown a difference in histone modification patterns between functional binding sites and non-functional ones at yeast ORF for almost half of all TFs tested, we investigated whether there is a correlation between the histone modifications at the ORF and the TF bindings to the corresponding promoter.
This method records the fact of the TF binding to the oligonucleotide corresponding to a predicted site, thereby making it possible to set the threshold directly and restrict the false-positives [ 3].
The maximum response signals of protein binding to the GAG surface, corresponding to the plateaus of the respective sensorgrams, were used for Scatchard plot analysis and the calculation of equilibrium Kd values.
Overall, the PS11-scFvFc-CD8-gp41 and PS11-scFvFc-CD28-gp41 proteins were the most competent in binding the peptide and exhibited higher levels of peptide binding compared to the corresponding constructs without the envelope incorporation motifs (Figure 3b, compare lanes 8 and 10 to lanes 7 and 9, respectively).
Because the RNA fragments contained in our samples were amplified using poly(A) tail primers, the binding probability to the corresponding microarray probes was maximized.
More suggestions(17)
settings to the corresponding
applicable to the corresponding
binding to the active
binding to the mammalian
binding to the upper
binding to the bacterial
binding to the AMP-lid
binding to the imprinted
binding to the degenerate
binding to the wild-type
binding to the cellular
binding to the extracellular
binding to the hydroxyapatite
binding to the 16-base
binding to the human
binding to the nucleobase
binding to the urinary
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com