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Background binding was subtracted from the value obtained for binding to the consensus DNA sequence.
ATP binding to the consensus NBS yields an intermediate with two ATP molecules bound ('1' in Figure 8A).
Zinc fingers 4 and 5, responsible for binding to the consensus sequence [ 27], are highly conserved specifically in the residues involved in contact with bases or phosphates.
Collectively, these data indicate that the IRF6-DBD possesses specific, high affinity binding to the consensus sequence AACCGAAACC/T in vitro.
As shown in Fig. 5, IRF-1 binding to the consensus element was induced after IFN-γ stimulation of ESTDAB-159 and of control cell line ESTDAB-056.
Red colonies (100%) were obtained, which indicated that this mutant p53 could not transactivate the Ade2 gene by binding to the consensus site from the RGC.
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To assess whether the EMT-inducing transcription factors can directly bind to the consensus binding elements present in the promoters of ABC transporters, we undertook a chromatin immunoprecipitation (ChIP) assay with MCF7 cells transiently transfected with pCDNA3-Flag-Twist construct.
Electrophoresis mobility shift assay (EMSA) further demonstrated that AnCrpA could bind to the consensus CRP binding site in E. coli [ 27].
It does so by binding to the degenerate consensus site WTGNNARCGNWWWCAW [1].
As shown in Fig. 6B, a mutation of binding site 1 almost completely abolished the activity of miR-222, whereas a mutation of binding site 2 only slightly reduced miR-222 activity, indicating that the majority of miR-222 activity is due to the binding to the first consensus sequence.
As shown in figure 2A, wild type competitor duplex, but not mutant NF-κB competitor duplex was able to prevent RelA/p65 from binding to the attached consensus sequence.
More suggestions(15)
binding to the colchicine
binding to the A-site
binding to the waaA
binding to the junction
binding to the lipid
binding to the brain
binding to the cognate
binding to the promoter
binding to the soil
binding to the enzyme
binding to the surface
binding to the receptor
binding to the sugar
binding to the gold
binding to the β-globin
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