Sentence examples for binding to the column from inspiring English sources

The amplicons binding to the column were eluted in water and the OD determined prior to adding appropriate amounts to tubes containing either left or right primer.

Though soluble expression of the His-tagged NTD increased very slightly using 12-litre cultures, the now-relatively-soluble hexahistidine tagged protein did not bind to the Ni-NTA column possibly due to an altered pattern of folding in the absence of the CTD, which possibly caused the hexa-histidine tag to get buried and therefore unavailable for binding to the column.

After binding to the column and washing, the RNA was eluted in 50 μl elution buffer (water, PCR grade).

The serum was diluted 1 1 with PBS, 10 mM EDTA was added, and loaded onto the OmCI-coupled column to allow C5 binding to the column.

As can be seen, the extent of binding to the column matrix (E1 and E2) is comparable to that observed with the rc and β controls.

4) Figure 3A and B. The binding of TTR to the rc and the β peptides is explained by the background binding to the column material.

By comparing the flow through (FT2) and elution fractions (E1/E2) from the avidin column, we estimated that 40% of the PfBCCP-79 from the metal chelate column was binding to the avidin column.

While the column-binding assay is not quantitative, nor was it expected to be, we can estimate the confidence in the peak densities by seeing to what extent TTR binds to the column matrix in the absence of coupled peptides.

Briefly, splenocytes were incubated with anti-CD25 antibody coupled to magnetic beads for 15 min prior to binding to the MACS column.

Another improvement is the use of synthetic eTHF as an internal standard to account for any loss of folate during extraction and subsequent binding to the affinity column.

The S10 extract was then complemented with 200 µg/mL of an RNA poly-C homopolymer (GE Lifesciences) to adsorb nonspecific binding proteins and reduce background binding to the RNA affinity column.